Year: 2022 | Month: September | Volume 15 | Issue 3
Biochemical and Molecular Characterization of Sweet Sorghum Varieties and Hybrids
U.S. Dalvi
P.U. Barve
M.S. Shinde and R.M. Naik
DOI:10.30954/0974-1712.03.2022.14
Abstract:
The present investigation entitled, “Biochemical and molecular characterization of sweet sorghum varieties and hybrids” was undertaken to study the sugar and juice quality parameters of promising sweet sorghum varieties and hybrids for the ultimate ethanol production. Genetic diversity of these varieties and hybrids were also studied using ISSR primers. Fifteen promising sweet sorghum varieties and three hybrids were grown in Kharif 2017 at All India Co-ordinated Sorghum Improvement Project, Mahatma Phule Krishi Vidyapeeth, Rahuri. The experiment was laid out in Randomized Block Design with three replications. The juice from stalks of each variety and hybrid was extracted and analyzed for 0brix, reducing sugar, non-reducing sugar, total sugar, and juice yield. The juice yield ranged from 4011-10661 l/ha with highest 10661 l/ha in sweet sorghum hybrid RSSH 50. The total sugar content in the stem juice of sweet sorghum varieties and hybrids varied from 11.01 to 13.47, with a mean value of 12.18 percent. Seven ISSR primers were used for the genetic diversity studies of sweet sorghum varieties and hybrids. The consensus tree divided ten varieties into three major clusters. One sub-cluster included five sweet sorghum varieties namely RSSV 517, RSSV 520, RSSV 542, CSV 19 SS, SSV 84 and one hybrid CSH 22 SS, while another sub cluster included three varieties viz., RSSV 527, RSSV 533 and RSSV 540.
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Highlights
- As per the present investigation during kharif season sweet sorghum genotype RSSV 542 and RSSV 522 are suitable for further breeding programme because they recorded maximum juice yield as well as total sugars which ultimately gives higher ethanol recovery.
- The diversity analysis of eight sweet sorghum varieties and two hybrids by ISSR primers revealed that RSSV 540 and RSSV 517 to be diverse genotypes and could be used for juice quality parameter improvement programme.
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